MBT 201- IPR & BIOSAFETY
Types of IP: Patents, Trademarks, Copyright & Related Rights, Industrial Design, Traditional Knowledge, Geographical Indications, Protection of GMOs IP as a factor in R&D; IPs of relevance to Biotechnology
History of GATT & TRIPS Agreement; Madrid Agreement; Hague Agreement; WIPO Treaties; Budapest Treaty; PCT; Indian Patent Act 2005 & recent amendments.
Introduction to Patents; Types of patent applications: Ordinary, PCT, Conventional, Divisional and Patent of Addition; Specifications: Provisional and complete; Forms and fees Invention in context of “prior art”; Patent databases; Searching International Databases; Country-wise patent searches (USPTO,esp@cenet (EPO), PATENT Scope (WIPO), IPO, etc.)
National & PCT filing procedure; Time frame and cost; Status of the patent applications filed; Precautions while patenting – disclosure/non-disclosure; Financial assistance for patenting - introduction to existing schemes. Patent licensing and agreement Patent infringement- meaning, scope, litigation, Deliberate testing-Transgenic testing, Centers in India for Testing.
Introduction; Principles of laboratory biosafety and biosecurity; Risk assessment: Occupational health hazards and laboratory associated infections (LAIs); Laboratory biosafety level criteria and biosafety laboratory design; Primary containment for biohazards: Selection, installation and used of biosafety cabinets; Decontamination and disinfection; Safe working practices in biorisk areas and waste management of biohazards; Biosafety guidelines - Government of India; Definition of GMOs & LMOs; Institutional Biosafety Committee (IBSC), Institutional Animal
Ethics Committee (IAEC), Review Committee for Genetically Modified organisms (RCGM), Genetic Engineering Approval Committee (GEAC) etc. for GMO applications in food and agriculture; Environmental release of GMOs. Handling and transportation of infectious material and genetically modified products.
http://www.wipo.int/portal/index.html.en
http://www.ipr.co.uk/IP_conventions/patent_cooperation_treaty.html
http://www.cbd.int/biosafety/background.shtml
http://www.cdc.gov/OD/ohs/symp5/jyrtext.htm
http://web.princeton.edu/sites/ehs/biosafety/biosafetypage/section3.html
MBT202-BIO-ENTREPRENEURSHIP
Taking decision on starting a venture; Assessment of feasibility of a given venture/new venture; Approach a bank for a loan; Sources of financial assistance; Making a business proposal/Plan for seeking loans from financial institution and Banks; Funds from bank for capital expenditure and for working; Statutory and legal requirements for starting a company/venture; Budget planning and cash flow management; Basics in accounting practices: concepts of balance sheet, P&L account, and double entry bookkeeping; Estimation of income, expenditure, profit, income tax etc.
Assessment of market demand for potential product (s) of interest; Market conditions, segments; Prediction of market changes; Identifying needs of customers including gaps in the market, packaging the product; Market linkages, branding issues; Developing distribution channels; Pricing/Policies/Competition; Promotion/Advertising; Services Marketing
With financiers, bankers etc.; with government/law enforcement authorities; With companies/Institutions for technology transfer; Dispute resolution skills; External environment/changes; Crisis/ Avoiding/Managing; Broader vision–Global thinking
How to use IT for business administration; Use of IT in improving business performance; Available software for better financial management; E-business setup, management.
Leadership skills; Managerial skills; Organization structure, pros & cons of different structures; Team building, teamwork; Appraisal; Rewards in small scale set up.
Support mechanism for entrepreneurship in India
Knowledge centres like universities and research institutions; Role of technology and up gradation; Assessment of scale of development of Technology; Managing Technology Transfer; Regulations for transfer of foreign technologies; Technology transfer agencies.
Texts/References:
MBT 203- IMMUNO-TECHNOLOGY
Module I Introduction
Immunology- fundamental concepts and anatomy of the immune system, Immune memory, Immune tolerance; Phagocytosis; Complement and Inflammatory responses; Haematopoesis; Mucosal Immunity; Antigens - immunogens, haptens; Major Histocompatibility Complex - MHC genes, MHC and immune responsiveness and disease susceptibility, HLA typing.
Module II
Hybridoma Technology
Hybridoma techniques and monoclonal Ab production- myeloma cell lines, fusion of myeloma cell lines with Ab producing B cells , fusion methods, selection and screening methods, for positive hybrids, -cloning methods- production and purification and characterization of MAb. Application of MAb in biomedical research, in clinical diagnosis and treatment. Production of human MAb and their applications. Production of polyclonal Ab with different type of Ag: Ag preparation and modification, adjuvants, dose and route of Ag administration,collection of sera.
Module III
Immunotechniques-Antigen-Antibody interactions
Immunoprecipitation- mancini method, ouchterloney method, immune electrophoresis, rocket immunoelectrophoresis, crossed immunoelectrophoresis, agglutination and complement mediated immune reactions; Advanced immunological techniques - RIA, ELISA, Western blotting, ELISPOT and ELAST assay, peptide based immuno binding assay, peptide mapping, epitope mapping, flurosence and photo illuminance based immunoassay, DELPHIA and SLFIA, concept of immunohisto / cyto-chemistry, immunofluorescence, flow cytometry and immunoelectron microscopy; detection of molecules in living cells, in situ localization by techniques such as FISH and GISH. Surface plasmon resonance, Biosenor assays for assessing ligand–receptor interaction, CMI techniques- lymphoproliferation assay, Mixed lymphocyte reaction, Cell Cytotoxicity assays, Apoptosis, Microarrays, Transgenic mice, Gene knock outs.
Module IV
New generation Vaccines: Immunobiotechnology
Vaccines and Vaccination, types of vaccines including new generation vaccines. Tumor immunology. Active and passive immunization; Live, killed, attenuated, sub unit vaccines; Vaccine technology- Role and properties of adjuvants, recombinant DNA and protein based vaccines, plant-based vaccines, reverse vaccinology; Peptide vaccines, conjugate vaccines; Antibody genes and antibody engineering- chimeric and hybrid monoclonal antibodies; Catalytic antibodies and generation of immunoglobulin gene libraries.
Module V
Clinical Immunology
T-cell cloning-importance of Ag presentation and MHC class II molecules in T-cell cloning, Ag specific and alloreactive T-cell cloning, use of T-cell cloning – cell cloning in understanding the immunologically relevant Ag and T-cell epitopes, application of T cell cloning in vaccine development. Hypersensitivity – Type I-V; Autoimmunity; Types of autoimmune diseases; Mechanism and role of CD4+ T cells; MHC and TCR in autoimmunity; Treatment of autoimmune diseases; Transplantation – Immunological basis of graft rejection; Clinical transplantation and immunosuppressive therapy; Tumor immunology – Tumor antigens; Immune response to tumors and tumor evasion of the immune system,Cancerimmunotherapy;Immunodeficiency-Primary immunodeficiencies, Acquired or secondary immunodeficiencies.
Case studies.
Lab o n Immunology:
Single Radial Immuno Diffusion.
Double diffusion.
Rocket Immunoelectrophoresis.
Counter- Current Immunoelectrophoresis.
Characterization of Immunoglobulins by SDS-PAGE.
Antibody titre by ELISA method.
Isolation of Lymphocytes from human blood and Culture
Culturing and maintenance of cell lines
Immunoblotting, Dot blot assays.
Abs Production.
Texts/References:
MBT 204 -STEM CELL TECHNOLOGY
Stem Cells: Definition, Classification and Sources, Blastocyst Culture, Xeno-free Derivation and Cryopreservation, Properties and Applications of Embryonic Stem Cells, Characterization of Human Embryonic Stem Cells, Stem Cells and their Developmental Potential. Culture, Subcloning, Spontaneous and Controlled Differentiation of Human Embryonic Stem Cells, In Vivo and In Vitro Differentiation of Human Embryonic Stem Cells, Feeder-free Culture of Human Embryonic Stem Cells,
Generation and Expansion of pluripotent Stem cells, Epigenesis in Pluripotent Cells, Organogenesis, Mammalian Nuclear Transfer Technology, Novel Strategies for the mobilization of HSC, GMP : clinical scale production of MSCs,
Stem Cell therapy for Neurodegenrative Diseases: Parkinson’s, Alzheimer, Spinal Cord injuries and other brain syndromes, Tissue system failures, Diabetic Cardiomycopathy, kidney failure, liver failure, Leukaemia, transplantation.
Purification and characterization of cancer stem cells, therapeutic implications of cancer stem cells: Preventative and therapeutic strategies for cancer stem cells, Targeting acute myelogenous leukaemia stem cells, targeting cancer stem cell pathways: Hedgehog/GLI signalling, Notch signalling pathway, Wnt.
Human Stem cells research: ethical considerations, stem cell religion consideration, Stem Cell based therapies: pre clinical regulatory consideration and patient advocacy, Intellectual property issues surrounding Human Embryonic Stem cells study.
Laboratory Exercise
Preparation of feeder cell culture.
Culturing of Stem cell line.
Isolation of cells from blastocyst.
Growing mesenchymal stem cell.
Preservation of cord blood.
Stem cell isolation from umbilical cord.
Types of cleavage, invertebrates, vertebrates,and permanent preparations – stains of stages of blastula
Development of Amphibian – Gestulation – Metamorphosis
Sex determination – Drosophila (Prescribed assignments with problems in genetic)
Stem cells –Identification of cells by staining of bone marrow – (Animal example)
Texts/References:
MBT 205 - PROTEOMICS & PROTEIN ENGINEERING
Architecture of Proteins: Amino acids, classification of proteins, Protein Structure: primary, secondary, tertiary and quaternary. Protein folding, thermodynamics and kinetics of protein folding, Protein Stability: protein stability & associated factors. Detection, identification and quantification of amino acids and proteins, In-silico protein modeling.
Charting protein–protein interactions: Topoisomerase-based cloning, Univector plasmid-fusion system, Two-hybrid analysis protein-protein interactions in yeast, viral, bacteria systems. Use of phage display to detect protein-ligand interactions, Detecting interactions by protein fragment complementation assays.
Outline of bioengineering of macromolecules a multidisciplinary approach; Methods to alter primary structure of protein: site directed mutagenesis; examples of engineered proteins, protein design, principles and examples. Steps involved in protein engineering and protein modeling to the desired needs.
Physical methods of determining the three-dimensional structure of proteins (X-ray crystallography, Nuclear magnetic resonance spectroscopy, Cryoelectron microscopy, Neutron diffraction, Optical spectroscopic techniques, Vibrational spectroscopy, Raman spectroscopy),
Use of 2-D PAGE, sensitivity and resolution and representation of 2-D gels, multiplexed analysis to show expression profiles; circular dichroism (CD), MALDITOF and MALDITOFTOF, special strategies for qualitative and quantitative analysis. Protein array for expressional analysis, profiling and functional analysis, application of proteomics to medicine.
Lab o n Protein Engineering
Using absorbance coefficients and extinction coefficients to estimate protein concentration
Protein quantitation when contaminating nucleic acids are present
Measuring protein concentration by colorimetric assay - the Bradford assay
The nitric acid method for protein estimation in biological samples
Quantitation of Tryptophan in Proteins
The CAT (chloramphenicol acetyltransferase) Assay
Use of Luciferase in a reporter assay
In vitro translation - Determining amino acid incorporation
Casting Immobilized pH Gradients (IPGs)
Carboxymethylation of cysteine using iodoacetamide/iodoacetic acid
Analyzing Protein Phosphorylation
Texts/References:
MBT 201- IPR & BIOSAFETY
Types of IP: Patents, Trademarks, Copyright & Related Rights, Industrial Design, Traditional Knowledge, Geographical Indications, Protection of GMOs IP as a factor in R&D; IPs of relevance to Biotechnology
History of GATT & TRIPS Agreement; Madrid Agreement; Hague Agreement; WIPO Treaties; Budapest Treaty; PCT; Indian Patent Act 2005 & recent amendments.
Introduction to Patents; Types of patent applications: Ordinary, PCT, Conventional, Divisional and Patent of Addition; Specifications: Provisional and complete; Forms and fees Invention in context of “prior art”; Patent databases; Searching International Databases; Country-wise patent searches (USPTO,esp@cenet (EPO), PATENT Scope (WIPO), IPO, etc.)
National & PCT filing procedure; Time frame and cost; Status of the patent applications filed; Precautions while patenting – disclosure/non-disclosure; Financial assistance for patenting - introduction to existing schemes. Patent licensing and agreement Patent infringement- meaning, scope, litigation, Deliberate testing-Transgenic testing, Centers in India for Testing.
Introduction; Principles of laboratory biosafety and biosecurity; Risk assessment: Occupational health hazards and laboratory associated infections (LAIs); Laboratory biosafety level criteria and biosafety laboratory design; Primary containment for biohazards: Selection, installation and used of biosafety cabinets; Decontamination and disinfection; Safe working practices in biorisk areas and waste management of biohazards; Biosafety guidelines - Government of India; Definition of GMOs & LMOs; Institutional Biosafety Committee (IBSC), Institutional Animal
Ethics Committee (IAEC), Review Committee for Genetically Modified organisms (RCGM), Genetic Engineering Approval Committee (GEAC) etc. for GMO applications in food and agriculture; Environmental release of GMOs. Handling and transportation of infectious material and genetically modified products.
http://www.wipo.int/portal/index.html.en
http://www.ipr.co.uk/IP_conventions/patent_cooperation_treaty.html
http://www.cbd.int/biosafety/background.shtml
http://www.cdc.gov/OD/ohs/symp5/jyrtext.htm
http://web.princeton.edu/sites/ehs/biosafety/biosafetypage/section3.html
MBT202-BIO-ENTREPRENEURSHIP
Taking decision on starting a venture; Assessment of feasibility of a given venture/new venture; Approach a bank for a loan; Sources of financial assistance; Making a business proposal/Plan for seeking loans from financial institution and Banks; Funds from bank for capital expenditure and for working; Statutory and legal requirements for starting a company/venture; Budget planning and cash flow management; Basics in accounting practices: concepts of balance sheet, P&L account, and double entry bookkeeping; Estimation of income, expenditure, profit, income tax etc.
Assessment of market demand for potential product (s) of interest; Market conditions, segments; Prediction of market changes; Identifying needs of customers including gaps in the market, packaging the product; Market linkages, branding issues; Developing distribution channels; Pricing/Policies/Competition; Promotion/Advertising; Services Marketing
With financiers, bankers etc.; with government/law enforcement authorities; With companies/Institutions for technology transfer; Dispute resolution skills; External environment/changes; Crisis/ Avoiding/Managing; Broader vision–Global thinking
How to use IT for business administration; Use of IT in improving business performance; Available software for better financial management; E-business setup, management.
Leadership skills; Managerial skills; Organization structure, pros & cons of different structures; Team building, teamwork; Appraisal; Rewards in small scale set up.
Support mechanism for entrepreneurship in India
Knowledge centres like universities and research institutions; Role of technology and up gradation; Assessment of scale of development of Technology; Managing Technology Transfer; Regulations for transfer of foreign technologies; Technology transfer agencies.
Texts/References:
MBT 203- IMMUNO-TECHNOLOGY
Module I Introduction
Immunology- fundamental concepts and anatomy of the immune system, Immune memory, Immune tolerance; Phagocytosis; Complement and Inflammatory responses; Haematopoesis; Mucosal Immunity; Antigens - immunogens, haptens; Major Histocompatibility Complex - MHC genes, MHC and immune responsiveness and disease susceptibility, HLA typing.
Module II
Hybridoma Technology
Hybridoma techniques and monoclonal Ab production- myeloma cell lines, fusion of myeloma cell lines with Ab producing B cells , fusion methods, selection and screening methods, for positive hybrids, -cloning methods- production and purification and characterization of MAb. Application of MAb in biomedical research, in clinical diagnosis and treatment. Production of human MAb and their applications. Production of polyclonal Ab with different type of Ag: Ag preparation and modification, adjuvants, dose and route of Ag administration,collection of sera.
Module III
Immunotechniques-Antigen-Antibody interactions
Immunoprecipitation- mancini method, ouchterloney method, immune electrophoresis, rocket immunoelectrophoresis, crossed immunoelectrophoresis, agglutination and complement mediated immune reactions; Advanced immunological techniques - RIA, ELISA, Western blotting, ELISPOT and ELAST assay, peptide based immuno binding assay, peptide mapping, epitope mapping, flurosence and photo illuminance based immunoassay, DELPHIA and SLFIA, concept of immunohisto / cyto-chemistry, immunofluorescence, flow cytometry and immunoelectron microscopy; detection of molecules in living cells, in situ localization by techniques such as FISH and GISH. Surface plasmon resonance, Biosenor assays for assessing ligand–receptor interaction, CMI techniques- lymphoproliferation assay, Mixed lymphocyte reaction, Cell Cytotoxicity assays, Apoptosis, Microarrays, Transgenic mice, Gene knock outs.
Module IV
New generation Vaccines: Immunobiotechnology
Vaccines and Vaccination, types of vaccines including new generation vaccines. Tumor immunology. Active and passive immunization; Live, killed, attenuated, sub unit vaccines; Vaccine technology- Role and properties of adjuvants, recombinant DNA and protein based vaccines, plant-based vaccines, reverse vaccinology; Peptide vaccines, conjugate vaccines; Antibody genes and antibody engineering- chimeric and hybrid monoclonal antibodies; Catalytic antibodies and generation of immunoglobulin gene libraries.
Module V
Clinical Immunology
T-cell cloning-importance of Ag presentation and MHC class II molecules in T-cell cloning, Ag specific and alloreactive T-cell cloning, use of T-cell cloning – cell cloning in understanding the immunologically relevant Ag and T-cell epitopes, application of T cell cloning in vaccine development. Hypersensitivity – Type I-V; Autoimmunity; Types of autoimmune diseases; Mechanism and role of CD4+ T cells; MHC and TCR in autoimmunity; Treatment of autoimmune diseases; Transplantation – Immunological basis of graft rejection; Clinical transplantation and immunosuppressive therapy; Tumor immunology – Tumor antigens; Immune response to tumors and tumor evasion of the immune system,Cancerimmunotherapy;Immunodeficiency-Primary immunodeficiencies, Acquired or secondary immunodeficiencies.
Case studies.
Lab o n Immunology:
Single Radial Immuno Diffusion.
Double diffusion.
Rocket Immunoelectrophoresis.
Counter- Current Immunoelectrophoresis.
Characterization of Immunoglobulins by SDS-PAGE.
Antibody titre by ELISA method.
Isolation of Lymphocytes from human blood and Culture
Culturing and maintenance of cell lines
Immunoblotting, Dot blot assays.
Abs Production.
Texts/References:
MBT 204 -STEM CELL TECHNOLOGY
Stem Cells: Definition, Classification and Sources, Blastocyst Culture, Xeno-free Derivation and Cryopreservation, Properties and Applications of Embryonic Stem Cells, Characterization of Human Embryonic Stem Cells, Stem Cells and their Developmental Potential. Culture, Subcloning, Spontaneous and Controlled Differentiation of Human Embryonic Stem Cells, In Vivo and In Vitro Differentiation of Human Embryonic Stem Cells, Feeder-free Culture of Human Embryonic Stem Cells,
Generation and Expansion of pluripotent Stem cells, Epigenesis in Pluripotent Cells, Organogenesis, Mammalian Nuclear Transfer Technology, Novel Strategies for the mobilization of HSC, GMP : clinical scale production of MSCs,
Stem Cell therapy for Neurodegenrative Diseases: Parkinson’s, Alzheimer, Spinal Cord injuries and other brain syndromes, Tissue system failures, Diabetic Cardiomycopathy, kidney failure, liver failure, Leukaemia, transplantation.
Purification and characterization of cancer stem cells, therapeutic implications of cancer stem cells: Preventative and therapeutic strategies for cancer stem cells, Targeting acute myelogenous leukaemia stem cells, targeting cancer stem cell pathways: Hedgehog/GLI signalling, Notch signalling pathway, Wnt.
Human Stem cells research: ethical considerations, stem cell religion consideration, Stem Cell based therapies: pre clinical regulatory consideration and patient advocacy, Intellectual property issues surrounding Human Embryonic Stem cells study.
Laboratory Exercise
Preparation of feeder cell culture.
Culturing of Stem cell line.
Isolation of cells from blastocyst.
Growing mesenchymal stem cell.
Preservation of cord blood.
Stem cell isolation from umbilical cord.
Types of cleavage, invertebrates, vertebrates,and permanent preparations – stains of stages of blastula
Development of Amphibian – Gestulation – Metamorphosis
Sex determination – Drosophila (Prescribed assignments with problems in genetic)
Stem cells –Identification of cells by staining of bone marrow – (Animal example)
Texts/References:
MBT 205 - PROTEOMICS & PROTEIN ENGINEERING
Architecture of Proteins: Amino acids, classification of proteins, Protein Structure: primary, secondary, tertiary and quaternary. Protein folding, thermodynamics and kinetics of protein folding, Protein Stability: protein stability & associated factors. Detection, identification and quantification of amino acids and proteins, In-silico protein modeling.
Charting protein–protein interactions: Topoisomerase-based cloning, Univector plasmid-fusion system, Two-hybrid analysis protein-protein interactions in yeast, viral, bacteria systems. Use of phage display to detect protein-ligand interactions, Detecting interactions by protein fragment complementation assays.
Outline of bioengineering of macromolecules a multidisciplinary approach; Methods to alter primary structure of protein: site directed mutagenesis; examples of engineered proteins, protein design, principles and examples. Steps involved in protein engineering and protein modeling to the desired needs.
Physical methods of determining the three-dimensional structure of proteins (X-ray crystallography, Nuclear magnetic resonance spectroscopy, Cryoelectron microscopy, Neutron diffraction, Optical spectroscopic techniques, Vibrational spectroscopy, Raman spectroscopy),
Use of 2-D PAGE, sensitivity and resolution and representation of 2-D gels, multiplexed analysis to show expression profiles; circular dichroism (CD), MALDITOF and MALDITOFTOF, special strategies for qualitative and quantitative analysis. Protein array for expressional analysis, profiling and functional analysis, application of proteomics to medicine.
Lab o n Protein Engineering
Using absorbance coefficients and extinction coefficients to estimate protein concentration
Protein quantitation when contaminating nucleic acids are present
Measuring protein concentration by colorimetric assay - the Bradford assay
The nitric acid method for protein estimation in biological samples
Quantitation of Tryptophan in Proteins
The CAT (chloramphenicol acetyltransferase) Assay
Use of Luciferase in a reporter assay
In vitro translation - Determining amino acid incorporation
Casting Immobilized pH Gradients (IPGs)
Carboxymethylation of cysteine using iodoacetamide/iodoacetic acid
Analyzing Protein Phosphorylation
Texts/References: